Journal: The Journal of Neuroscience
Article Title: Ciliary Neurotrophic Factor (CNTF) Enhances Myelin Formation: A Novel Role for CNTF and CNTF-Related Molecules
doi: 10.1523/JNEUROSCI.22-21-09221.2002
Figure Lengend Snippet: The promyelinating effect of CNTF is signaled by gp130-containing receptors. A, Cultures were treated with IL-6, the IL-6-soluble receptor α subunit (sR-IL-6), or IL-6 +sR-IL-6 at either 10 or 50 ng/ml each. Results are expressed as a percentage of control β-galactosidase levels (means ± SEM of 3 independent experiments with 4–6 different cultures per experiment). B, CNTF (10 ng/ml) and JAK inhibitor AG490 (AG) were added daily into the culture medium between 14 and 18 DIV, and β-galactosidase was assayed at 19 DIV. β-galactosidase levels are expressed as means ± SEM (6 different culture wells for each condition) of one representative experiment. ***p < 0.001; Student'st test.
Article Snippet: Human recombinant IL-6, IL-11, oncostatin M (OsM), and human recombinant soluble α-chain of IL-6 receptor were supplied by Chemicon (Temecula, CA).
Techniques: